RNA

Part:BBa_K3467000:Design

Designed by: Wang Ran   Group: iGEM20_CAU_China   (2020-10-20)


2X pepper RNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has a high melting temperature. Also, this part is commonly employed in E.coli and mammalian cell.


Source

This part sequence comes from the design from professor Yi Yang's lab of East China University of Science and Technology. We bought the part 2X pepper RNA standard sample from FD Biotech company.

References

Chen, X. et al., Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. NAT BIOTECHNOL 37 1287 (2019).